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E8751 | CAS:1239-45-8 | EB | EtBr | Ethidium bromide | 溴化乙锭
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E8751 Ethidium bromide
Sigma-Aldrich ~95% (HPLC)
窗体底端
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货号
号 |
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您的价格
RMB |
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E8751-1G |
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301.86 |
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E8751-5G |
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1,157.13 |
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E8751-10G |
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2,007.72 |
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E8751-25G |
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4,814.55 |
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Synonym: |
3,8-Diamino-5-ethyl-6-phenylphenanthridinium bromide, EtBr, Homidium bromide |
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CAS Number: |
1239-45-8 |
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Empirical Formula (Hill Notation): |
C21H20BrN3 |
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Molecular Weight: |
394.31 |
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Beilstein Registry Number: |
3642536 |
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EC Number: |
214-984-6 |
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MDL number: |
MFCD00011724 |
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PubChem Substance ID: |
24894683 |
Description
| Frequently Asked Questions |
Live Chat and Frequently Asked Questions are available for this Product. |
| Application |
Ethidium bromide (EtBr) is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/ml). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids.1,2,3 It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA.4 Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase.5 |
| Biochem/physiol Actions |
Ethidium bromide intercalates double-stranded DNA and RNA and acts as a frameshift mutagen. It can also be used in conjunction with acridine orange to differentiate between viable, apoptotic and necrotic cells. |
| Reconstitution |
For staining a gel after electrophoresis, dilute a sample of the stock solution to 0.5 μg/ml with water and incubate the gel for 15-30 min. Destaining is usually not needed but can be carried out in water for 15 min if decreased background is necessary. The DNA bands can then be detected on a UV light box (254 nm wavelength). Ethidium bromide can also be incorporated into the gel and running buffer at 0.5 μg/ml and visualized immediately after electrophoresis. |
性质
| assay |
~95% (HPLC) |
| mp |
260-262 °C (dec.)(lit.) |
安全性
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