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D9542 | CAS:28718-90-3 | DAPI | 4′,6-Diamidino-2-phenylindole dihydrochloride

D9542    4′,6-Diamidino-2-phenylindole dihydrochloride

Sigma          powder, ≥98% (HPLC and TLC)

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货号
号

您的价格
RMB

D9542-1MG

278.46

D9542-5MG

758.16

D9542-10MG

1,457.82

D9542-50MG

4,399.20

Synonym:

2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride, DAPI dihydrochloride

CAS Number:

28718-90-3

Empirical Formula (Hill Notation):

C16H15N5 · 2HCl

Molecular Weight:

350.25

Beilstein Registry Number:

4894417

EC Number:

249-186-7

MDL number:

MFCD00012681

PubChem Substance ID:

24894305

Description

Frequently Asked Questions Live Chat and Frequently Asked Questions are available for this Product.
Biochem/physiol Actions Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.
Caution Protect from light.
Application DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.

性质

assay ≥98% (HPLC and TLC)
form powder
fluorescence λex 340 nm; λem 488 nm (nur DAPI)
  λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)
EQP level Premium
solubility H2O: soluble20 mg/mL (heat or sonication may be required. Solutions stored in the dark at room temperature or 4 °C should be stable for 2 to 3 weeks.)
  PBS: insoluble
abs. ε1mM/263 nm, H2O 30
storage temp. 2-8°C

安全性

Personal Protective Equipment Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
Hazard Codes Xi
Risk Statements 36/37/38
Safety Statements 26-36
WGK Germany 3

参考文献

reference Naimski, P., Quantitative fluorescent analysis of different conformational forms of DNA bound to the dye, 4', 6-diamidine-2-phenylindole, and separated by gel electrophoresis. Anal. Biochem. 106, 471, (1980)
  Otto, F.J., High-resolution analysis of nuclear DNA employing the fluorochrome DAPI. Methods Cell Biol. 41, 211-217, (1994)
  Kapuscinski, J., DAPI: a DNA-specific fluorescent probe. Biotech. Histochem. 70, 220-233, (1995)
  Darzynkiewicz, Z. and Li, X., Cotter, T.G. and Martin, S.J., ed., Measurements of cell death by flow cytometry, in Techniques in Apoptosis: A User's Guide. Cytometry London, U.K. , (1996), 91-92
  Uphoff, C.C., et al., Mycoplasma contamination in human leukemia cell lines. I. Comparison of various detection methods. J. Immunol. Methods 149, 43-53, (1992)
  Jeppesen, C.,, Nielsen, P.E, Photofootprinting of drug-binding sites on DNA using diazo- and azido-9-aminoacridine derivatives. Eur. J. Biochem. 182, 437, (1989)
  Zaitsev, E.N., Kowalczykowski, S.C., Binding of double-stranded DNA by Escherichia coli RecA protein monitored by a fluorescent dye displacement assay. Nucleic Acids Res. 26, 650-654, (1998)
  Collins, J.A., Major DNA fragmentation is a late event in apoptosis. J. Histochem. Cytochem. 45, 923, (1997)
  Hotz, M.A., et al., Flow cytometric detection of apoptosis: comparison of the assays of in situ DNA degradation and chromatin changes. Cytometry 15, 237-244, (1994)
  Uphoff, C.C., et al., Sensitivity and specificity of five different mycoplasma detection assays. Leukemia 6, 335-341, (1992)