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碳水化合物


43819 | CAS:3483-12-3 | DTT | DL-Dithiothreitol | 二硫苏糖醇

43819   DL-Dithiothreitol

Fluka         99.0% (RT)

窗体底端

货号

您的价格
RMB

43819-1G

476.19

43819-5G

1,184.04

43819-25G

4,774.77

Synonym:

threo-1,4-Dimercapto-2,3-butanediol, Clelands reagent, DTT

CAS Number:

3483-12-3

Linear Formula:

HSCH2CH(OH)CH(OH)CH2SH

Molecular Weight:

154.25

Beilstein Registry Number:

1719757

EC Number:

222-468-7

MDL number:

MFCD00004877

Description

Application An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).

性质

assay ≥99.0% (RT)
total impurities ≤0.3% 4,5-dihydroxy-1,2-dithiane
mp 41-44 °C(lit.)
  42-43 °C
solubility H2O: soluble50 mg/mL, clear, colorless
anion traces sulfate (SO42-): ≤50 mg/kg
cation traces Ca: ≤10 mg/kg
  Cd: ≤5 mg/kg
  Co: ≤5 mg/kg
  Cr: ≤5 mg/kg
  Cu: ≤5 mg/kg
  Fe: ≤5 mg/kg
  K: ≤50 mg/kg
  Mg: ≤5 mg/kg
  Mn: ≤5 mg/kg
  Na: ≤200 mg/kg
  Ni: ≤5 mg/kg
  Pb: ≤5 mg/kg
  Zn: ≤5 mg/kg
storage temp. 2-8°C

安全性

Personal Protective Equipment dust mask type N95 (US), Eyeshields, Faceshields, Gloves
Hazard Codes Xn
Risk Statements 22-36/37/38
Safety Statements 26-36
WGK Germany 3
RTECS EK1610000
Flash Point(F) 235.4 °F
Flash Point(C) 113 °C