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酶和辅酶 |
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C9891 | CAS:9001-12-1 | Collagenase IA | 胶原酶IA
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C9891 Collagenase from Clostridium histolyticum
Sigma Type IA, 0.5-5.0 FALGPA units/mg solid, ≥125 CDU/mg solid (CDU = collagen digestion units), essentially salt-free, lyophilized powder
窗体底端
货号 号 |
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您的价格 RMB |
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C9891-25MG |
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444.60 |
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C9891-100MG |
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789.75 |
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C9891-500MG |
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2,506.14 |
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C9891-1G |
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3,951.09 |
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C9891-5G |
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15,793.83 |
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Synonym: |
Clostridiopeptidase A |
CAS Number: |
9001-12-1 |
Enzyme Commission (EC) Number: |
3.4.24.3 ( BRENDA | IUBMB ) |
EC Number: |
232-582-9 |
MDL number: |
MFCD00130830 |
Description
Analysis Note |
Also contains clostripain, nonspecific neutral protease, and tryptic activities. |
Linkage |
Similar to Type I, but produced by Sigma. |
Other Notes |
View more information on collagenase and cell dissociation at www.sigma-aldrich.com/enzymeexplorer. |
Preparation Note |
Equivalent to first 40% ammonium sulfate fraction of Mandl, I., et al., J. Clin. Invest., 32, 1323 (1953). |
Unit Definition |
One collagen digestion unit liberates peptides from collagen equivalent in ninhydrin color to 1.0 μmole of leucine in 5 hr at pH 7.4 at 37 °C in the presence of calcium ions. One FALGPA hydrolysis unit hydrolyzes 1.0 μmole of furylacryloyl-Leu-Gly-Pro-Ala per min at pH 7.5 at 25 °C in the presence of calcium ions. One neutral protease unit hydrolyzes casein to produce color equivalent to 1.0 μmole tyrosine per 5 hr at pH 7.5 at 37 °C. One clostripain unit hydrolyzes 1.0 μmole of BAEE per min at pH 7.6 at 25 °C in the presence of DTT. |
性质
description |
For general use |
form |
essentially salt-free, lyophilized powder |
storage temp. |
−20°C |
安全性
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