L6876 | CAS:12650-88-3 | Lysozyme from chicken egg white | 溶菌酶

L6876  Lysozyme from chicken egg white Sigma      lyophilized powder, protein ≥90 %, ≥40,000 units/mg protein 窗体底端 货号 号 您的价格 RMB L6876-1G 562.77 L6876-5G 1,898.91 L6876-10G 2,551.77 L6876-25G 5,831.28 L6876-100G 20,780.37 Synonym: Mucopeptide N-acetylmuramoylhydrolase, Muramidase CAS Number: 12650-88-3 Enzyme Commission (EC) Number: 3.2.1.17  ( BRENDA | IUBMB ) EC Number: 235-747-3 MDL number: MFCD00131557 Description Frequently Asked Questions Live Chat and Frequently Asked Questions are available for this Product. Application Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts. Other Notes View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer Preparation Note 3× crystallized Unit Definition One unit will produce a ΔA450 of 0.001 per min at pH 6.24 at 25 °C, using a suspension of Micrococcus lysodeikticus as substrate, in a 2.6 mL reaction mixture (1 cm light path). Physical form Dialyzed and lyophilized, containing buffer salts as sodium acetate and sodium chloride Biochem/physiol Actions Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane. The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M). 性质 form lyophilized powder mol wt single-chain mol wt 14.3 kDa composition protein, ≥90% EQP level Premium storage temp. −20°C 安全性 Personal Protective Equipment Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter Safety Statements 22-24/25 WGK Germany 3 RTECS OL5989000