P6782 | CAS:9003-99-0 | Peroxidase from horseradish | Horseradish peroxidase | 辣根过氧化物酶

P6782  Peroxidase from horseradish Sigma       Type VI-A, essentially salt-free, lyophilized powder, ~1000 units/mg solid (using ABTS), 250-330 units/mg solid (using pyrogallol) 窗体底端 货号 号 目录价 RMB P6782-5MG 575.64 P6782-10MG 1,070.55 P6782-25MG 1,751.49 P6782-50MG P6782-100MG 5,288.40 Synonym: Donor:hydrogen-peroxide oxidoreductase, Horseradish peroxidase CAS Number: 9003-99-0 Enzyme Commission (EC) Number: 1.11.1.7  ( BRENDA | IUBMB ) EC Number: 232-668-6 MDL number: MFCD00071339 Description Analysis Note This product is assayed using ABTS for easy comparison to other suppliers′ unit: approx. 1,000 units per mg solid   The RZ (Reinheitszahl) is the absorbance ratio A403/A275 determined at 0.5-1.0 mg/ml in deionized water. It is a measure of hemin content, not enzymatic activity. Even preparations with high RZ may have low enzymatic activity. Linkage Similar to P8375 Other Notes View more information on peroxidase at www.sigma-aldrich.com/enzymeexplorer. Packaging Packaged in mg solid Unit Definition One ABTS unit will oxidize 1 μmole of ABTS per minute at 25 °C at pH 5.0   One pyrogallol unit will form 1.0 mg purpurogallin from pyrogallol in 20 sec at pH 6.0 at 20 °C. General description Horseradish peroxidase is isolated from horseradish roots (Amoracia rusticana) and belongs to the ferroprotoporphyrin group of peroxidases. HRP is a single chain polypeptide containing four disulfide bridges. It is a glycoprotein containing 18% carbohydrate. The carbohydrate composition consists of galactose, arabinose, xylose, fucose, mannose, mannosamine, and galactosamine depending upon the specific isozyme. Its molecular weight (~44 kDa) includes the polypeptide chain (33,890 Daltons), hemin plus Ca2+ (~700 Daltons), and carbohydrate (~9,400 Daltons). At least seven isozymes of HRP exist. The isoelectric point for horseradish peroxidase isozymes ranges from 3.0 - 9.0. 性质 type Type VI-A form essentially salt-free, lyophilized powder EQP level Premium storage temp. 2-8°C 安全性 Personal Protective Equipment Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter WGK Germany 3