P6911 | CAS:9036-06-0 | Pronase E | 蛋白酶E

P6911   Protease from Streptomyces griseus Sigma        BioReagent, for molecular biology, DNase, RNase, and nickase, none detected (No RNase.) 窗体底端 货号 号 您的价格 RMB P6911-100MG 522.99 P6911-1G 1,841.58 P6911-5G 6,362.46 Synonym: Actinase E, Pronase E CAS Number: 9036-06-0 EC Number: 232-909-5 MDL number: MFCD00132092 Description Application Protease is typically used in nucleic acid isolation procedures in incubations of 0.5-3.0 hours supplemented with 0.2% sodium dodecyl sulfate and 10 mM EDTA. Preparation Note Collected from culture broth of S. griseus and purified by successive column procedures. Specificity A mixture of at least three proteolytic activities including an extracellular serine protease. In general, serine proteases display a wide range of substrate specificities, which are believed to be mediated by an active site composed of one Asp, one His, and a Ser residue in the molecule. This enzyme prefers to hydrolyze peptide bonds on the carboxyl side of glutamic or aspartic acid. Unit Definition One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent). Physical properties Completely inactivated by heating above 80 °C for 15-20 minutes. 性质 grade for molecular biology product line BioReagent activity ≥4 units/mg solid mol wt monomer mol wt ~20 kDa solubility H2O: soluble5-20 mg/mL foreign activity DNase, RNase, and nickase, none detected (No RNase.) storage temp. −20°C 安全性 Personal Protective Equipment Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter Safety Statements 22-24/25 WGK Germany 2 RTECS UK9595000